|
KMID : 1134820100390060927
|
|
Journal of the Korean Society of Food Science and Nutrition
2010 Volume.39 No. 6 p.927 ~ p.931
|
|
|
Defatted Grape Seed Extracts Suppress Adipogenesis in 3T3-L1 Preadipocytes
|
|
Choi Young-Min
Lee Seon-Mi
Kim Young-Wha
Jeon Keon-Wook
Seong Ji-Hae
Jeong Heon-Sang
Lee Jun-Soo
|
|
|
Abstract
|
|
|
|
The objective of this study was to evaluate the effect of defatted grape seed extract (DGSE) on adipocyte differentiation in 3T3-L1 preadipocytes. DGSE at 100 ㎍/mL significantly suppressed lipid accumulation and glycerol-3-phosphate dehydrogenase activity in hormonally stimulated adipocytes, an indicator of adipocyte differentiation. In order to understand the anti-adipogenic effects of DGSE, the changes in the expression of several adipogenic transcription factors including peroxisome proliferator-activated receptor (PPAR) γ, CCAAT/ enhancer-binding protein (C/EBP) α and β were investigated using immunoblotting. DGSE suppressed the expression of PPARγ, C/EBPα, and C/EBPβ proteins compared with control adipocytes in a dose-dependent manner. This results indicated that DGSE may alter fat mass by directly affecting adipogensis in maturing preadipocytes and thus may have applications for the treatment of obesity.
|
|
|
KEYWORD
|
|
|
defatted grape seed extract, 3T3-L1, adipogenesis, PPARγ, C/EBP
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|